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RPMI-1640 Medium is a widely used cell culture medium designed for the growth of various types of human and animal cells. It is a complex mixture of nutrients, including amino acids, vitamins, salts, and glucose, which provide the essential components required for cell proliferation and maintenance in vitro. RPMI-1640 is particularly favored for its ability to support the growth of suspension cells and adherent cells alike.
| pH range | 6.8 to 7.4 |
| Osmolality | 280 to 320 mOsm/kg |
| Storage temperature | 2 to 8 degrees Celsius |
| Form | Liquid |
| Supplementation | May require additional supplements like serum or growth factors |
When selecting RPMI-1640 Medium, consider the specific cell types you will be culturing, as different cells may have unique requirements for supplementation. It's important to specify whether you need the medium with or without phenol red, as well as any specific formulations or additives required for your application. Additionally, inquire about the quantities needed, as bulk purchasing may offer cost savings.
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RPMI-1640 is suitable for a variety of cell types, including lymphocytes, hybridomas, and some adherent cell lines.
Yes, but additional supplements may be necessary to support cell growth.
It should be stored at 2 to 8 degrees Celsius and protected from light.
For long-term storage, it is recommended to aliquot and freeze the medium.
Typically, RPMI-1640 has a shelf life of 12 to 24 months when stored properly.
Yes, but the performance may vary depending on the specific primary cell type.